CD56bright
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Contents |
Introduction
Regulatory NK cell subset, mainly producing cytokines rather than killing of virus infected or cancer cells.
CD56bright NK cells and diseases
Activated (CD69+) CD56bright NK cells may play a role in liver inflammation in hepatitis C virus infected patients (Lin et al., 2004)
Katchar et al. (2005 [1]) found that the majority of lung NK cells in patients with pulmonary sarcoidosis are CD56bright. Most lung NK cells expressed HLA-E-specific inhibitory receptors (i.e. CD94/NKG2A), but only a few killer cell immunoglobulin-like inhibitory receptors specific for HLA-A/B/C.
Gene expression profiling CD56bright versus CD56 dim NK cells
Koopman et al. (2003, [2]) compared gene expression of decidual versus peripheral blood NK cells and included gene expression data of CD56bright versus CD56dim NK cell subsets. Genes are listed in the order of x-fold change of bright vs. dim cells. Significant differences are listed in bold.
- higher expression in bright cells
- RANK (12 fold), BMP2, CD9, IFITM3, HLA-DRA, ITGAX, CD44, KIAA1775, IL7R, CD2-AP, SPRY 2, SPRY1, C2GNT, Serpine1
- lower expression in bright cells
- Galectin-1, OSTF epsilon, TGFBR3, TP epsilon, CD6, SLC16A6, TNFR-II, GZM A (Granzyme A), KIR3DL2, KIR3DL1, KIR2DL3, RORA, GZM B, SCYA5, MIP-1A, MIP-1B, CD16 (22 fold)
Interestingly gene expression of CD56 is nearly identical although flow cytometry demonstrates a difference in protein level of 5 to 10 fold. CD56 is slightly higher expressed in mRNA of CD56bright cells.
Minimal differences in gene expression were identified for the following genes:
- CD63, CD56, NKG2C, CD59, NKG2E, DEGS, TSSC3
- RAC2, KIR2DL4, SLC9A6, DAD1, DEFCAP, HCS, DAG1, MPV17, CD53, NK4, FASL, CTNNA1, PRF 1 (Perforin)
Dzionek, Turkiewicz, Lokan et al. (2005) compared gene expression of MACS selected NK cell subsets using PIQOR immunology arrays. 11 genes (out of 1076 present on the array) were significantly differently expressed:
- RAR, TNFSF6, AIOLOS, MCP, CD9, CDH3, CCL3-CCl3L1, CCL5, NOTCH2, PECAM1, PRKCZ
Further Reading
References
2007
Chan, A., Hong, D.-L., Atzberger, A., Kollnberger, S., Filer, A. D., Buckley, C. D., McMichael, A., Enver, T., Bowness, P.
CD56bright Human NK Cells Differentiate into CD56dim Cells: Role of Contact with Peripheral Fibroblasts
Immunol 2007 179: p. 89-94
Romagnani, C., Juelke, K., Falco, M., Morandi, B., D'Agostino, A., Costa, R., Ratto, G., Forte, G., Carrega, P., Lui, G., Conte, R., Strowig, T., Moretta, A., Munz, C., Thiel, A., Moretta, L., Ferlazzo, G.
CD56brightCD16- Killer Ig-Like Receptor- NK Cells Display Longer Telomeres and Acquire Features of CD56dim NK Cells upon Activation
Immunol 2007 178: p. 4947-4955 NKref0536
2006
Chidrawar SM, Khan N, Chan TY, Nayak L, Moss PA.
Ageing is associated with a decline in peripheral blood CD56bright NK cells.
Immun Ageing. 2006 Nov 29;3(1):10. Epub, PMID:17134511
Bielekova B, Catalfamo M, Reichert-Scrivner S, Packer A, Cerna M, Waldmann TA, McFarland H, Henkart PA, Martin R.
Regulatory CD56bright natural killer cells mediate immunomodulatory effects of IL-2R{alpha}-targeted therapy (daclizumab) in multiple sclerosis.
Proc Natl Acad Sci U S A. 2006 Apr 3;. Epub, PMID:16585503
2005
Batoni G, Esin S, Favilli F, Pardini M, Bottai D, Maisetta G, Florio W, Campa M.
Human CD56dim and CD56bright Natural Killer Cell Subsets Respond Differentially to Direct Stimulation with Mycobacterium bovis Bacillus Calmette-Guerin.
Scand J Immunol. 2005 Dec;62(6):498-506. PMID: 16316416
Increased Susceptibility to Apoptosis of CD56dimCD16+ NK Cells Induces the Enrichment of IFN-{gamma}-Producing CD56bright Cells in Tuberculous Pleurisy
Schierloh, P., Yokobori, N., Aleman, M., Musella, R. M., Beigier-Bompadre, M., Saab, M. A., Alves, L., Abbate, E., de la Barrera, S. S., Sasiain, M. C.
J Immunol 2005 175: p. 6852-6860
Li Z, Lim WK, Mahesh SP, Liu B, Nussenblatt RB.
Cutting Edge: In Vivo Blockade of Human IL-2 Receptor Induces Expansion of CD56bright Regulatory NK Cells in Patients with Active Uveitis.
J Immunol. 2005 May 1;174(9):5187-91. PMID: 15843513
van den Heuvel MJ, Horrocks J, Bashar S, Hatta K, Burke S, Evans SS, Croy BA, Tekpetey FR.
Peri-ovulatory Increases in Tissue Homing Potential of Circulating CD56bright Cells Are Associated With Fertile Menstrual Cycles.
J Clin Endocrinol Metab. 2005 Mar 22; Epub PMID: 15784713
Freud AG, Becknell B, Roychowdhury S, Mao HC, Ferketich AK, Nuovo GJ, Hughes TL, Marburger TB, Sung J, Baiocchi RA, Guimond M, Caligiuri MA.
A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cells.
Immunity. 2005 Mar;22(3):295-304. PMID: 15780987
Differential expression of SHIP1 in CD56bright and CD56dim NK cells provides a molecular basis for distinct functional responses to monokine costimulation
Trotta, R., Parihar, R., Yu, J., Becknell, B., Allard, J. II, Wen, J.,Ding, W., Mao, H., Tridandapani, S., Carson, W. E., Caligiuri, M. A.
Blood 2005 105: p. 3011-3018
NK cell cytokine secretion regulated by SHIP1
Ritz, J.
Blood 2005 105: p. 3003-3003
Characterisation of natural killer cells and [[CD56+ T-cell]]s in sarcoidosis patients
Katchar, K., Soderstrom, K., Wahlstrom, J., Eklund, A., Grunewald, J.
Eur Respir J 2005 26: p. 77-85
Bratke K, Kuepper M, Bade B, Virchow JC Jr, Luttmann W.
Differential expression of human granzymes A, B, and K in natural killer cells and during CD8(+) T cell differentiation in peripheral blood.
Eur J Immunol. 2005 Aug 16;. Epub PMID: 16106370 NKref0479
2004
Natural killer cell lytic activity and CD56dim and CD56bright cell distributions during and after intensive training
Suzui, M., Kawai, T., Kimura, H., Takeda, K., Yagita, H., Okumura, K., Shek, P. N., Shephard, R. J.
J. Appl. Physiol. 2004 96: p. 2167-2173
The IL-12 Signature: NK Cell Terminal [[CD56+high]] Stage and Effector Functions
Loza, M. J., Perussia, B.
J Immunol 2004 172: p. 88-96
Vitale M, Della Chiesa M, Carlomagno S, Romagnani C, Thiel A, Moretta L, Moretta A.
The small subset of CD56brightCD16- natural killer cells is selectively responsible for both cell proliferation and interferon-gamma production upon interaction with dendritic cells.
Eur J Immunol. 2004 Jun;34(6):1715-22. PMID: 15162442 NKref0312 (CD56bright, NK/DC interaction)
Lin AW, Gonzalez SA, Cunningham-Rundles S, Dorante G, Marshall S, Tignor A, Ha C, Jacobson IM, Talal AH
CD56(+dim) and CD56(+bright) cell activation and apoptosis in hepatitis C virus infection.
Clin Exp Immunol. 2004 Aug;137(2):408-16. PMID: 15270860
2003
Koopman LA, Kopcow HD, Rybalov B, Boyson JE, Orange JS, Schatz F, Masch R, Lockwood CJ, Schachter AD, Park PJ, Strominger JL.
Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential.
J Exp Med. 2003 Oct 20;198(8):1201-12. PMID: 14568979
2001
Human natural killer cells: a unique innate immunoregulatory role for the CD56bright subset
Cooper, M. A., Fehniger, T. A., Turner, S. C., Chen, K. S., Ghaheri, B. A., Ghayur, T., Carson, W. E., Caligiuri, M. A.
Blood 2001 97: p. 3146-3151
1997
Carson WE, Fehniger TA, Caligiuri MA.
CD56bright natural killer cell subsets: characterization of distinct functional responses to interleukin-2 and the c-kit ligand.
Eur J Immunol. 1997 Feb;27(2):354-60. PMID: 9045904
